humanmethylation27 bead array Search Results


humanmethylation27 bead array  (INFINIUM Inc)
90
INFINIUM Inc humanmethylation27 bead array
DNA-methylation profiles were analyzed with the <t>HumanMethylation27</t> BeadChip microarray which represents 27,578 unique CpG sites. MSC derived from adipose tissue (MSC-AT) were compared with those derived from bone marrow, which was either aspirated from the iliac crest (MSC-BM) or taken from the caput femuris upon hip replacement (MSC-Hip). Unsupervised principal component analysis (PCA) clearly separated DNA-methylation profiles according to the tissue of origin in the first dimension (PC1), whereas the forth component (PC4) discerned early and late passage ( A ). Scatterplot comparison of passage 5 and passage 10 in MSC-AT revealed that 233 CpG sites are more than 15% hyper-methylated whereas 186 CpG sites are more than 15% hypo-methylated at passage 10 ( B ). Significance Analysis of Microarray (SAM) was used to select 517 senescence-associated CpG sites (FDR = 4.8%) and these are presented as a heatmap ( C ; data were divided by the mean of each row for graphical presentation).
Humanmethylation27 Bead Array, supplied by INFINIUM Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/humanmethylation27+bead+array/pmc03227452-14-9-8?v=INFINIUM+Inc
Average 90 stars, based on 1 article reviews
humanmethylation27 bead array - by Bioz Stars, 2026-08
90/100 stars
  Buy from Supplier

Image Search Results


DNA-methylation profiles were analyzed with the HumanMethylation27 BeadChip microarray which represents 27,578 unique CpG sites. MSC derived from adipose tissue (MSC-AT) were compared with those derived from bone marrow, which was either aspirated from the iliac crest (MSC-BM) or taken from the caput femuris upon hip replacement (MSC-Hip). Unsupervised principal component analysis (PCA) clearly separated DNA-methylation profiles according to the tissue of origin in the first dimension (PC1), whereas the forth component (PC4) discerned early and late passage ( A ). Scatterplot comparison of passage 5 and passage 10 in MSC-AT revealed that 233 CpG sites are more than 15% hyper-methylated whereas 186 CpG sites are more than 15% hypo-methylated at passage 10 ( B ). Significance Analysis of Microarray (SAM) was used to select 517 senescence-associated CpG sites (FDR = 4.8%) and these are presented as a heatmap ( C ; data were divided by the mean of each row for graphical presentation).

Journal: Aging (Albany NY)

Article Title: Replicative senescence of mesenchymal stem cells causes DNA-methylation changes which correlate with repressive histone marks

doi:

Figure Lengend Snippet: DNA-methylation profiles were analyzed with the HumanMethylation27 BeadChip microarray which represents 27,578 unique CpG sites. MSC derived from adipose tissue (MSC-AT) were compared with those derived from bone marrow, which was either aspirated from the iliac crest (MSC-BM) or taken from the caput femuris upon hip replacement (MSC-Hip). Unsupervised principal component analysis (PCA) clearly separated DNA-methylation profiles according to the tissue of origin in the first dimension (PC1), whereas the forth component (PC4) discerned early and late passage ( A ). Scatterplot comparison of passage 5 and passage 10 in MSC-AT revealed that 233 CpG sites are more than 15% hyper-methylated whereas 186 CpG sites are more than 15% hypo-methylated at passage 10 ( B ). Significance Analysis of Microarray (SAM) was used to select 517 senescence-associated CpG sites (FDR = 4.8%) and these are presented as a heatmap ( C ; data were divided by the mean of each row for graphical presentation).

Article Snippet: Subsequently, we have compared DNA-methylation profiles with the Infinium HumanMethylation27 Bead Array and the profiles differed markedly in MSC derived from adipose tissue and bone marrow.

Techniques: DNA Methylation Assay, Microarray, Derivative Assay, Methylation